Skip to main content
SpringerLink
Log in

Primary culture of avian pancreatic acinar cells for heterologous gene expression

  • Letter to the Editor
  • Scientific Reports
  • Published:
In Vitro Cellular & Developmental Biology - Animal Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Akiyama, T.; Whitaker, B.; Federspeil, M., et al. Tissue-specific expression of mouse tyrosinase gene in cultured chick cells. Exp. Cell Res. 214: 154–162; 1994.

    Article  PubMed  CAS  Google Scholar 

  • Ali, S.; Hall, J.; Hazlewood, G. P.; Hirst, B. H.; Gilbert, H. J. A protein targeting signal that functions in polarised epithelial cells in vivo. Biochem. J. 315:857–862; 1996.

    PubMed  CAS  Google Scholar 

  • Ali, S.; Fontes, C. M. G. A.; Hazlewood, G. P., et al. Co-integration and expression of bacterial and genomic transgenes in the pancreatic and intestinal tissues of transgenic mice. Gene 202:203–208; 1997.

    Article  PubMed  CAS  Google Scholar 

  • Chomezynski, P.; Sacchi, N. Single step method of RNA isolation by guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156–159; 1987.

    Google Scholar 

  • Donovan, D. M.; Sax, C. M.; Klement, J. F., et al. Conservation of mouse αA-crystallin promoter activity in chicken lens epithelial cells. J. Mol. Evol. 35:337–345; 1992.

    Article  PubMed  CAS  Google Scholar 

  • Duncan, M. K.; Roth, J.; Thompson, M.; Kantorow, M.; Piatigorsky, J. Chicken β B1 crystallin: gene sequence and evidence for functional conservation of promoter activity between chicken and mouse. Biochim. Biophys. Acta 1261:68–76; 1995.

    PubMed  Google Scholar 

  • Frazier, L. M.; Longnecker, D. S. Cell lines of the human and rodent exocrine pancreas. In: Go, V. L. W.; Dimagno, E.P.; Gardner, J.D.; Lebenthal, E.; Reber, H.A.; Scheele, G. eds. The pancreas: biology, pathobiology and disease. 2nd ed. New York: Raven Press; 1993:565–574.

    Google Scholar 

  • Hall, J.; Ali, S.; Surani, M. A., et al. Manipulation of the repertoire of digestive enzymes secreted into the gastrointestinal tract of transgenic mice. Bio/Technology 11:376–379; 1993.

    Article  PubMed  CAS  Google Scholar 

  • Hall, J.; Hazlewood, G. P.; Barker, P. J.; Gilbert, H. J. Conserved reiterated domains in Clostridium thermocellum endoglucanases are not essential for catalytic activity. Gene 69:29–38; 1988.

    Article  PubMed  CAS  Google Scholar 

  • Jessop, N. W.; Hay, R. J. Characteristics of two rat pancreatic exocrine cell lines derived from transplantable tumours. In Vitro 16:212; 1980.

    Google Scholar 

  • Oda, D.; Savard, C. E.; Nguyen, T. D.; Swenson, E. R.; Lee, S. P. Culture of human main pancreatic duct epithelial cells. In Vitro Cell. Dev. Biol. 34:211–216; 1998.

    CAS  Google Scholar 

  • Ornitz, D. M.; Palmiter, R. D.; Hammer, R. E., et al. Specific expression of an elastase-human growth hormone fusion gene in pancreatic acinar cells of transgenic mice. Nature 313:600–612; 1985.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological and Nutritional Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne, UK

    Judith Hall, Ian R. Jowsey & Brian Brown

Authors
  1. Judith Hall
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ian R. Jowsey
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Brian Brown
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Judith Hall.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hall, J., Jowsey, I.R. & Brown, B. Primary culture of avian pancreatic acinar cells for heterologous gene expression. In Vitro Cell.Dev.Biol.-Animal 36, 290–292 (2000). https://doi.org/10.1290/1071-2690(2000)036<0290:PCOAPA>2.0.CO;2

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1290/1071-2690(2000)036<0290:PCOAPA>2.0.CO;2

Keywords

Search

Navigation